This project was aimed to develop smart phone based-electrochemical biosensors for analyzing steroid drugs especially hazardous estrogens (estrone E1, estradiol E2, estriol E3) and anti-estrogens (Bisphenol A) which can be easily applicable for domestic use as well as industrial purpose for monitoring of water samples. The developed sensing system can analyze the results in a few minutes with added advantages of throughput processing, improved quantification limits, reduced sample and reagent volume, enhanced sensitivity of detection and common platform for both sampling and detection. Overall, the combination of electrochemistry with biochemistry will provide new insight to better understanding of the interaction between steroids and bioreceptor materials.